Professeurs

Formation

Post-doctorat en oncologie moléculaire, Université d'Ottawa Post-doctorat en biologie cellulaire et moléculaire, Texas A&M University Doctorat en physiologie et endocrinologie, Université Laval Maîtrise en physiologie et endocrinologie, Université Laval Baccalauréat en biochimie, Université Laval

Chaire de recherche du Canada en gynéco-oncologie moléculaire; Régulation cellulaire et moléculaire de la mort cellulaire; Survie des cellules; Cancers du sein, de l'utérus et de l'ovaire; Thérapie génique; Chimiothérapie; Physiologie de la reproduction; Implantation embryonnaire; Grossesse et menstruations; Infertilité.

Formation

• B.Sc. biologie, concentration physiologie animale (U. Laval)
• M.Sc. médecine expérimentale (U. Laval)
• Ph.D. sciences pharmaceutiques (U.Laval)
• Postdoctorat en métabolisme du cancer (U. Ottawa)

La chimiothérapie est le principal traitement utilisé pour traiter les cancers du sein avancés. Malheureusement, une proportion importante de tumeurs développe une résistance à la chimiothérapie, impactant négativement la survie des patientes. Afin de contrer ce phénomène, mon laboratoire s’intéresse aux altérations métaboliques supportant le développement de la résistance au traitement dans la tumeur. Notre but est d’identifier et d’exploiter des vulnérabilités métaboliques présentes durant le développement de la résistance et dans les cellules résistantes.

1. Minarrieta L., Annis M.G., Audet-Delage Y., Kuasne H., Pacis A., St-Louis C., Nowakowski A., Biondini M., Khacho M., Park M., Siegel P.M., and St-Pierre J. (2024). Mitochondrial elongation impairs breast cancer metastasis. Science Advances 10, eadm8212(2024). DOI: 10.1126/sciadv.adm8212
2. Biondini M., Lehuédé C., Tabariès S., Annis M.G., Pacis A., Ma E.H., Tam C., Hsu B.E., Audet-Delage Y., Abu-Thuraia A., Girondel C., Sabourin V., Totten S.P., De Sa Tavares Russo M., Bridon G., Avizonis D., Guiot M.-C., St-Pierre J., Ursini-Siegel J., Jones R., and Siegel P.M. Metastatic Breast Cancer Cells are Metabolically Reprogrammed to Maintain Redox Homeostasis during Metastasis. Redox Biology. 75, 103276. DOI: 10.1016/j.redox.2024.103276.
3. Audet-Delage Y., St-Louis C., Minarrieta L., McGuirk S., Kurreal I., Annis M.G., Mer A.S., Siegel P.M., and St-Pierre J. (2023). Spatiotemporal modeling of chemoresistance evolution in breast tumors uncovers dependencies on SLC38A7 and SLC46A1. Cell Reports. 42 (10). DOI: 10.1016/j.celrep.2023.113191
4. Audet-Delage Y., Rouleau M., Villeneuve L., and Guillemette C. (2022). The Glycosyltransferase Pathway: An Integrated Analysis of the Cell Metabolome. Metabolites. 12 (10), 1006.
5. McGuirk S., Audet-Delage Y., Annis M.G., Xue Y., Vernier M., Zhao K., St-Louis C., Minarrieta L., Patten D.A., Morin G., Greenwood C.M.T., Giguère V., Huang S., Siegel P.M., and St-Pierre J. (2021). Resistance to different anthracycline chemotherapeutics elicits distinct and actionable primary metabolic dependencies in breast cancer. eLife. 10, e65150.
6. McGuirk S., Audet-Delage Y., and St-Pierre J. (2020) Metabolic Fitness and Plasticity in Cancer Progression. Trends in Cancer 6, 49-61. Co-premier auteur. 

• Chercheur-boursier junior 1, FRQS, 2025-2029
• Bourse pour la relève scientifique, Société de recherche sur le cancer, 2023-2026
• Bourse Michael W. McBurney pour chercheur postdoctoral, Université d'Ottawa, 2024
• Bourse du meilleur article scientifique pour un chercheur postdoctoral, Institut de biologie des système d'Ottawa, 2024
• Bourse de formation postdoctorale, FRQS, 2019-2021 et 2022-2023
• Distinction au Tableau d'honneur, Université Laval, 2019

Formation

Post-doctorat en rétrovirologie, Columbia University, New York, USA. Post-doctorat en rétrovirologie, University of Virginia, Charlottesville, USA. Doctorat en virologie, École Normale Supérieure, Lyon, France.

rétrovirologie, virologie, biologie moléculaire, biologie cellulaire, microbiologie, immunologie, maladies infectieuses

Formation

Formation

Helmholtz Munich, Allemagne - Postdoctorat (fellowship CIHR) - 2020
Centre de recherche du CHUM, Montréal, Canada - Postdoctorat (fellowship Diabetes Canada) - 2017
Université Laval, Québec, Canada - Doctorat (fellowship CIHR) - 2013

Neurobiologie de l'obésité

Nutrition

Neurosciences

Sélection:

I González-García, A Cebrian-Serrano, RE Contreras, E García-Clavé, O Le Thuc, Y Xu, T Gruber, B Legutko, J Lintelmann, J Adamski, TD Müller, SC Woods, P Pfluger, MH Tschöp, A Fisette#*, Cristina García-Cáceres#*. *Co-auteur senior, #Auteur co-correspondant. 2022. Estradiol regulates leptin sensitivity to control feeding via hypothalamic Cited1. Cell Metabolism

C Quarta*, A Fisette*, Y Xu, G Colldén, B Legutko, YT Tseng, A Reim, M Wierer, MC De Rosa, V Klaus, R Rausch, VV Thaker, E Graf, TM Strom, AL Poher, T Gruber, O Le Thuc, A Cebrian-Serrano, D Kabra, L Bellocchio, SC Woods, GO Pflugfelder, R Nogueiras, L Zeltser, IC Grunwald Kadow, A Moon, C García-Cáceres, M Mann, M Treier, CA Doege, MH Tschöp. *Co-premier auteur. 2019. “Functional identity of hypothalamic melanocortin neurons depends on Tbx3.” Nature Metabolism

A Fisette*, D Sergi *, A Breton-Morin, S Descôteaux, MG Martinoli. *Co-premier auteur. 2022. “New insights on the role of bioactive food derivatives in neurodegeneration and neuroprotection.” Current Pharmaceutical Design

A Fisette, S Tobin, L Décarie-Spain, K Bouyakdan, ML Peyot, SRM Madiraju, M Prentki, S Fulton, T Alquier. 2016. “α/β-hydrolase domain 6 in the ventromedial hypothalamus controls energy metabolism flexibility.” Cell Reports

L Décarie-Spain*, A Fisette*, Z Zhu, B Yang, RD DiMarchi, MH. Tschöp, B Finan, S Fulton, C Clemmensen. *Co-premier auteur. 2019. “GLP-1/Dexamethasone inhibits food reward without inducing mood and memory deficits in mice.” Neuropharmacology

Liste complète: https://pubmed.ncbi.nlm.nih.gov/?term=fisette%20a 

Formation

Post-doctorat en Neurosciences, Université d'Ottawa

Post-doctorat en Immunologie, UBC

Doctorat en Biochimie, McGill University

Maitrise en Biologie Cellulaire et Moléculaire, Université Laval

Bacc. en Biochimie, Université Laval

Mitochondries, métabolisme, mort cellulaire, maladies neurodégénératives

1. Ilamathi HS*, Benhammouda S*, Desrochers-Goyette J*, Lines MA, Germain M. (2022). DRP1 regulates endoplasmic reticulum sheets to control mitochondrial DNA replication and segregation. iScience, in revision
2. Lounas A, Lebrun A, Laflamme I, Vernoux N, Savage J, Tremblay, M-E, Germain M, Richard F. (2022). Mitochondrial morphology in porcine cumulus cells: a 3D perspective. Scientific Reports. 12:15403: 1-10.
3. Bilen M, Benhammouda S, Slack RS, Germain M. (2022). The integrated stress response as a key pathway downstream of mitochondrial dysfunction. Current Opinion in Physiology. 28:100555: 1-12.
4. Tlili M, Acevedo H, Descoteaux A, Germain M, Heinonen KM. (2022). Cell- intrinsic Wnt4 ligand regulates mitochondrial oxidative phosphorylation in macrophages. Biol. Chem.298:102193: 1-15.
5. Ilamathi HS*, Ouellet M*, Sabouny R, Desrochers- Goyette J*, Lines MA, Pfeffer G, Shutt TE, Germain (2021). Mitomate Tracker: an automated tool to quantify nucleoid distribution. Sci. Rep. 11:22755: 1-14.
6. Benhammouda S*, Vishwakarma A*, Gatti P*, Germain M*. (2021). Mitochondria Endoplasmic Reticulum Contact Sites (ERMCS): Tools to study organelle interaction. Frontiers in Cell Dev. Biol. 9:789959
7. Klionski D et al. (2021). Guidelines for the use and interpretation of assays for monitoring autophagy (Fourth Edition) [The authors include a large number of labs working on autophagy (including my lab) by alphabetical order]. Autophagy. 17: 1-382.
8. Amari L*, Germain M. (2021). Mitochondrial Extracellular Vesicles – Origins and Roles. Front. Mol. Neurosci. 14:767219.
9. Todkar K*, Chikhi L*, Desjardins V*, El-Mortada F*, Pépin G, Germain M. (2021). Selective packaging of mitochondrial proteins into extracellular vesicles prevents the release of mitochondrial DAMPs. Nature Communications. 12:1971: 1-12.
10. Costa G, Caputi FF, Serra M, Simola N, Rullo L, Stamatakos S, Sanna F, Germain M, Martinoli MG, Candeletti S, Morelli M, Romualdi P. (2021). Activation of Antioxidant and Proteolytic Pathways in the Nigrostriatal Dopaminergic System After 3,4-Methylenedioxymethamphetamine Administration: Sex-Related Differences. Front. Pharmacol.12:713486: 1-16.
11. Gatti P*, Ilamathi HS*, Todkar K*, Germain M. (2020). Mitochondria Targeted Viral Replication and Survival Strategies—Prospective on SARS-CoV-2. Front. Pharmacol.11:578599: 1-9.
12. Todkar K*, Chikhi L*, Germain M. (2019). Mitochondrial interaction with the endosomal compartment in endocytosis and mitochondrial transfer. Mitochondrion. 49: 284-288.
13. Almutawa W, Smith C, Sabouny R, Smit RB, Zhao T, Wong R, Lee-Glover L, Desrochers-Goyette J*, Ilamathi HS*, Care4Rare Canada Consortium, Suchowersky O, Germain M, Mains PE, Parboosingh JS, Pfeffer G, Innes AM, Shutt TE. (2019). The R941L mutation in MYH14 disrupts mitochondrial fission and causes peripheral neuropathy. EBioMedicine. 45: 379-392.
14. Lounas A*, Vernoux N, Germain M, Tremblay ME, Richard FJ. (2019). Mitochondrial sub-cellular localization of cAMP-specific phosphodiesterase 8A in ovarian follicular cells. Sci. 9:12493: 1-10.
15. Patten DA, Ouellet M*, Allan DS, Baird S, Germain M, Harper ME, Richardson, R. (2018). Mitochondrial adaptation following ionizing radiation in human mesenchymal stem cells. FASEB J. May 21:fj201801483RR: 9263-9278.
16. Ouellet M*, Guillebaud G*, Gervais V* Lupien St-Pierre D, Germain M. (2017). A novel algorithm identifies stress-induced alterations in mitochondrial connectivity and innermembrane structure from confocal images. PLoS Computational Biology. 13(6): e1005612.
17. St-Cyr Giguère F, Attiori Essis S, Chagniel L, Germain M, Cyr M, Massicotte G. (2017). The sphingosine-1-phosphate receptor 1 agonist SEW2871 reduces Tau-Ser262 phosphorylation in rat hippocampal slices. Brain Research. 1658: 51-59.
18. Todkar K*, Ilamathi HS*, Germain M. (2017). Mitochondria and Lysosomes: Discovering Bonds. Frontiers in Cell and Developmental Biology. 5(Art. 106): 1-7.
19. Achour I, Arel-Dubeau AM, Renaud J, Legrand M, Attard E, Germain M, Martinoli MG. (2016). Oleuropein Prevents Neuronal Death, Mitigates Mitochondrial Superoxide Production and Modulates Autophagy in a Dopaminergic Cellular Model. International Journal of Molecular Sciences. 17: pii: E1293.
20. Khacho M, Clark A, Svoboda DS, Azzi J, MacLaurin J, Meghaizel C, Sesaski H, Lagace DC, Germain M, Harper ME, Park DS, Slack RS. (2016). Mitochondrial dynamics directs the identity and self-renewal capacity of stem cells by regulating a nuclear transcriptional program. Cell Stem Cell. 19(2): 232-247.
21. Demers-Lamarche J*, Guillebaud G*, Todkar K*, Tlili M*, Bélanger N*, Nguyen AP, Grondin M*, Michel J, Germain M. (2016). Loss of mitochondrial function impairs lysosomes. J. Biol. Chem. 291(19): 10263-10276.
22. Germain M. (2015). OPA1 and mitochondrial solute carriers in bioenergetic metabolism. Molecular and Cellular Oncology. 2(2): e982378.
23. Patten DA*, Wong J, Khacho M, Soubannier V, Mailloux RJ, Pilon-Larose K, MacLaurin J, Park DS, McBride H, Trinkle-Mulcahy L, Harper ME, Germain M†, Slack RS† (†Co-corresponding authors). (2014). OPA1 senses metabolic changes through SLC25 family proteins to regulate mitochondrial structure and function. EMBO J. 33(22): 2676-2691.
24. Arel-Dubeau AM, Longpré F, Bournival J, Tremblay C, Demers-Lamarche J*, Haskova P, Attard E, Germain M, Martinoli MG. (2014). Cucurbitacin E has neuroprotective properties and autophagic modulating activities on dopaminergic neurons. Oxidative Medicine and Cellular Longevity. 425496: 1-15.

Formation

• 2020-2023 : Post-doctorat en physiologie musculaire (Université de Padoue / IR-CUSM et Institut Vénitien de Médecine Moléculaire)
• 2016-2020 : Ph.D. en Physiologie et physiopathologie musculaire (Université McGill)
• 2014-2016 : M. Sc. en Physiologie mitochondriale (Université du Québec à Montréal)
• 2011-2014 : B. Sc. en Kinésiolgie/physiologie de l'exercice (Université du Québec à Montréal)

- Mécanismes de l'atrophie musculaire

- Maladies métaboliques

- Biologie mitochondriale

- Vieillissement musculaire (sarcopénie) 

- Maladies neuromusculaires

- Physiologie de l'exercice

- Physiologie et physiopathologie musculaire

QUELQUES PUBLICATIONS

Sélection:

1. Desjardins K, Taherkhani S, Méthot L, Castonguay V, Leduc-Gaudet JP. (2024) Insights into the influence of high-intensity interval training on skeletal muscle clock gene expression in insulin-resistant individuals. Journal of physiology https://doi.org/10.1113/JP286939 
2. Roussel MP, Ravel-Chapuis A, Gobin J, Jasmin BJ, Leduc-Gaudet JP, Gagnon C, Duchesne E. (2024) Changes in Physiopathological Markers in Myotonic Dystrophy Type 1 Skeletal Muscle: A 3-Year Follow-up Study. Journal of Neuromuscular Diseases
3. Sedraoui S, Leduc-Gaudet JP, Mayaki D, Moamer A, Huck L, Gouspillou G, Petrof B, Hussain SNA. (2024) Lack of compensatory mitophagy in skeletal muscles during sepsis. Journal of physiology https://doi.org/10.1113/JP286216
4. Girard-Côté L, Gallais B, Gagnon Cynthia, Roussel MP, Morin M, Hébert LJ, Monckton D, Leduc-Gaudet JP, Gouspillou G, Macangelli V, Duchesne E. (2024) Resistance training in women with DM1: a multisystemic therapeutic avenue. Neuromuscular Disorders https://doi.org/10.1016/j.nmd.2024.05.009
5. Cefis M, Dargegen M, Marcangeli V, Taherkhani S, Dulac M, Leduc-Gaudet JP, Mayaki D, Hussain SNA, (2024) Gouspillou G. MFN2 overexpression in skeletal muscles of young and old mice causes a mild hypertrophy without altering mitochondrial respiration and H₂O₂ emission.  Acta Physiologica https://doi.org/10.1111/apha.14119
6. Leduc-Gaudet JP#, Miguez K, Cefis M, Faitg J, Moamer A, Chaffer TJ, Reynaud O, Broering FE, Shams A, Mayaki D, Huck L, Sandri M, Gouspillou G#, Hussain SNA#. Autophagy ablation in skeletal muscles worsens sepsis-induced muscle wasting, impairs whole-body metabolism, and decreases survival. #Auteur correspondant. (2023) IScience https://www.sciencedirect.com/science/article/pii/S2589004223015523 
7. Leduc-Gaudet JP, Guirguis C, Sincennes MC. Emerging links between metabolism and epigenetic regulation of muscle stem cells. (2023) Med Sci (Paris) PMID: 37387661
8. Franco-Romero A*,Leduc-Gaudet JP*, Hussain SNA, Gouspillou G, Sandri S. PHAF1/MYTHO is a novel autophagy regulator that controls muscle integrity. *Co-premier auteur. (2023) Autophagy PMID: 37309183
9. Leduc-Gaudet JP*, Franco-Romero A*, Broering F, Cefis M, Moamer A, et al. MYTHO: a novel regulator of skeletal muscle autophagy and integrity. *Co-premier auteur. (2023) Nature Communications PMID:36864049
10. Reynaud O, Wang J, Ayoub MB, Leduc-Gaudet JP, Mayaki D, Dulac M, Hussain S, Bergeron R, and Gouspillou G. The impact of high fat feeding and Parkin overexpression on skeletal muscle mass, mitochondrial respiration and H₂O₂ (2022) American Journal of Physiology-Cell Physiology PMID:36571445
11. Leduc-Gaudet JP, Hussain SNA, Gouspillou G, (2022) Parkin: A potential target to promote healthy aging. The Journal of physiology PMID:36571445
12. Gusev E, Liang F, Bhattarai S, Broering F, Leduc-Gaudet JP, Hussain SNA, Radzioch D, and Petrof B. (2022) Characterization of skeletal muscle wasting pathways in diaphragm and limb muscles of cystic fibrosis mice. American Journal of Physiology-Regulatory, Integrative and Comparative Physiology PMID:35411814
13. Leduc-Gaudet JP and Hussain SNA, (2022) Muscle wasting in COPD: Not enough autophagy? American Journal of Respiratory Cell and Molecular Biology PMID:35294850
14. Chaffer T, Cefis M, Leduc-Gaudet JP, Circadian control of skeletal muscle adaptations to exercise. The Journal of physiology PMID:34339519
15. Leduc-Gaudet JP#, Hussain SNA, Barreiro E, and Gouspillou G. (2021) Mitochondrial Dynamics and Mitophagy in Skeletal Muscle Health and Aging. #Auteur correspondant. International Journal of Molecular Sciences PMID:34360946
16. Dulac M, Leduc-Gaudet JP, Cefis M, Ayoub MB, Reynaud O, Shams A, et al. (2021) Regulation of muscle and mitochondrial health by the mitochondrial fission protein Drp1 in aged mice. The Journal of physiology PMID:34269418 
17. Leduc-Gaudet JP, Miguez K, Cefis M, Moamer A, Chaffer TJ, Faitg J, et al. Role of autophagy in sepsis-induced skeletal muscle dysfunction, whole-body metabolism, and survival. (2021) bioRxiv
18. Klionsky, D. J., (...) Leduc-Gaudet JP, (...) et al. (2021) Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition). Autophagy PMID:33634751 
19. Leduc-Gaudet JP, Mayaki D, Reynaud O, Broering F, Chaffer T, Hussain SNA and Gouspillou G.(2020) Parkin Overexpression Attenuates Sepsis-Induced Muscle Wasting. Cells PMID: 32545383
20. Dulac M, Leduc-Gaudet JP, Reynaud O, Ayoub M.B, Guérin A, Finkelchtein M, et al. (2020). Drp1 knockdown induces severe muscle atrophy and remodelling, mitochondrial dysfunction, autophagy impairment and denervation. The Journal of physiology PMID: 32539155
21. Janice Sanchez B, Tremblay, AK, Leduc-Gaudet JP, Hall DT, Kovacs E, Ma JF, Mubaid S, Hallauer, et al. (2019). Depletion of HuR in murine skeletal muscle enhances exercise endurance and prevents cancer-induced muscle atrophy. Nature Communication PMID: 31519904
22. Leduc-Gaudet JP*, Reynaud O*, Hussain SNA, Gouspillou G. (2019) Parkin overexpression protects from aging-related loss of muscle mass and strength. *Co-premier auteur. The Journal of Physiology PMID:30614532
23. Faitg J, Leduc-Gaudet JP, Reynaud O, Ferland G, Gaudreau P, Gouspillou G. (2019) Effects of aging and caloric restriction on mitochondrial morphology and dynamics in rat oxidative and glycolytic muscles. Frontiers in Physiology PMID: 31114501
24. Moroz N, Maes K, Leduc-Gaudet JP, Goldberg P, Petrof BJ, Mayaki D. et al. (2019) Oxidant regulation of muscle proteolytic pathways in the diaphragm during prolonged mechanical ventilation. Anesthesiology PMID: 31408447
25. Leduc-Gaudet JP, Reynaud O, Chabot F, Mercier J, Andrich DE, St-Pierre DH, Gouspillou G. (2018) The impact of a short-term high-fat diet on mitochondrial respiration, reactive oxygen species production, and dynamics in oxidative and glycolytic skeletal muscles of young rats. Physiological Reports PMID: 29479852
26. St-Jean-Pelletier F, Pion CH, Leduc-Gaudet JP, Sgarioto N, Zovile I, Barbat-Artigas S, Reynaud O, Alkaterji F, Lemieux FC, Grenon A, Gaudreau P, Hepple RT, Chevalier S, Belanger M, Morais JA, Aubertin-Leheudre M, Gouspillou G. (2017) The impact of ageing, physical activity, and pre-frailty on skeletal muscle phenotype, mitochondrial content, and intramyocellular lipids in men. The Journal of Cachexia, Sarcopenia and Muscle PMID: 27897402
27. Stana F, Vujovic M, Mayaki D, Leduc-Gaudet JP, Leblanc P, Huck L, Hussain SNA. (2017) Differential Regulation of the Autophagy and Proteasome Pathways in Skeletal Muscles in Sepsis. Critical Care Medicine PMID: 28538438   
28. Leduc-Gaudet JP, Picard M, St-Jean Pelletier F, Sgarioto N, Auger MJ, Vallée J, Robitaille R, St-Pierre DH, Gouspillou G. (2015) Mitochondrial morphology is altered in atrophied skeletal muscle of aged mice. Oncotarget PMID: 26053100

Liste complète: https://pubmed.ncbi.nlm.nih.gov/?term=Leduc-Gaudet+JP&cauthor_id=28538438&size=50 

2024: Récipiendaire d'une subvention à la découverte du CRSNG

2024: Récipiendaire du Discovery Award 2024 de la Banting Research Foundation

2023: NMD4C Biomedical Publication of the Year award

2021: FRQS Postdoctoral Fellowship

2020: Journal of Physiology Early Investigator Prize 2019 - Exercise and Muscle

2016: Vanier Canada Graduate Scholarship (CIHR)

2015: Lieutenant Governor’s Youth Medal (Bronze) 

2015: FRQS Master’s Research Scholarship

2014: Frederick Banting and Charles Best Canada Graduate Scholarship

2014: Strathcona Trust Plaque

Formation

Stage postodoctoral en Neurologie, McGill University, Montreal, Canada, 1993-1994

Stage postdoctoral en Genetique et Biologie Moléculaire, Harvard University, Boston, MA, USA , 1991-1993

Doctorat en Physiologie-Endocrinologie, Université Laval, Québec, Canada.

Laurea en Sciences Biologiques (Neurobiologie), Université de Turin (Italie)

Neurobiologie, neuroprotection, neurodégénerescence, phytoestrogènes, phytostérols, steroïdes, maladie de Parkinson.

Neurobiology, neuroprotection, neurodegeneration, phytoestrogens, phytosterols, steroids, Parkinson's disease.

Formation

Formation

Formation

Formation

Formation

Formation

Stage postdoctoral en biophysique et biologie cellulaire, UQTR Doctorat en immunologie-virologie, INRS-Institut Armand-Frappier, Canada Maîtrise en biophysique, UQTR

Cancer, infertilité, biologie cellulaire, biologie moléculaire, signalisation intracellulaire, facteurs de transcription