I obtained this protocol from Yuelin Zhang's group at the National Institute of Biological Sciences (NIBS, China). It has been published in the MOS7 paper (get the full reference from the Publications page):
Cheng Y.*, Germain H.*, Wiermer M.*, Bi D.*, Xu F., García A., Wirthmueller L., Després C., Parker J., Zhang Y., and Li X. 2009. Nuclear Pore Complex Component MOS7/Nup88 Is Required for Innate Immunity and Nuclear Accumulation of Defense Regulators in Arabidopsis. THE PLANT CELL
Note that all steps must be carried on ice
Note that all steps must be carried on ice
20 mM | Tris-HCl pH 7.4 |
25% | Glycerol |
20 mM | KCl |
2 mM | EDTA |
2,5 mM | MgCl2 |
250 mM | Sucrose |
Add 1 mM PMSF just before use.
20 mM | Tris-HCl pH 7.4 |
25% | Glycerol |
2,5 mM | MgCl2 |
0.2% | Triton X-100 |