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Génomique fonctionnelle des phytopathosystèmes

KIDS-DNA extraction


Fruit such as bananas
Table Salt
Baking soda
Liquid dishsoap
Rubbing alcohol
Cheese cloth (or coffee filter)
Tooth picks or paper clip
Ice bucket


  1. Place alcohol in ice bucket and keep for later
  2. Prepare “DNA extraction solution”: add 1 tsp baking soda, 1 tsp dish soap and ¼ tsp salt to ½ cup of water in a becher.
  3. Put 1 1-inch slice of fruit in a small bowl and smash with a spoon or mortar and pestle.
  4. Add 3-4 tbsp “DNA extraction solution” to the bowl and grind with the spoon until the fruit is well pulverized.
  5. Place the cloth on top of a narrow becher. Filter the DNA extract.
  6. Add an equal volume of the cold rubbing alcohol, mix by swirling. Wait ~ 5 min. or more.
  7. “Fish” out precipitated DNA with a paper clip hook.