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Génomique fonctionnelle des phytopathosystèmes

Soil selection of transgenics using phosphinothricin/glufosinate/Basta


  1. Weigh between 0.1 and 0.2 gram of seeds and vernalize in water for 48h.
  2. Resuspend seeds in 200ml water, using a 10 ml pipette dispense seeds on a flat.
  3. Place flat in growth room with a clear lid for a week.
  4. Remove lid.
  5. Spray 3-time weekly with 15 mg/L of herbicide.
  6. Resistant plant will appear after 2 weeks.
  7. These can either be transfer to a new flat or spraying can continue until all non-reistant plant die.