This protocol is derived from:
Clough, S.J., and Bent, A.F. (1998). Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana. The Plant Journal 16, 735-743.
however we observed that transformation efficiency could be increased when Silwet L-77 is substituted by OFX-309
Mireault, C., Paris, L.-P., Germain, H. 2014. Enhancement of the Arabidopsis floral dip method with XIAMETER OFX-0309 as alternative to Silwet L-77 surfactant. Botany. 92:1-3
5g/l | beef-extract |
1g/l | yeast-extract |
5g/l | peptone |
5g/l | sucrose |
2 mM | MgSO4 |