Total protein extraction

For Western from plant tissue

1. Grind sample to a fine powder with liquid nitrogen
2. Add two volume of extraction buffer, keep on ice 5 minutes
3. Spin at 13krpm/10 minutes
4. Transfer sup a clean tube
5. Repeat spin once and transfer sup to a clean tube
6. Mix with protein loading buffer and boil 5 minutes.

When protein extracts are stored in the freezer before being loaded they should be warmed up at 40C for 2-3 minutes to re-solubilize the SDS.

**Protein amount can be quantified with Bradford.