Protocols

These are useful protocols that I have used over the years. Some of them are not my own. Please take care in giving credits to whom it belongs.

• Arabidopsis thaliana nuclei purification protocol
• Desalting superquick protocol
• Floral dipping
• Soil selection of transgenics using phosphinothricin/glufosinate/Basta
• Strategy to make triple mutant and multiple mutants
• Generating single insertion homozygous tDNA lines in Arabidopsis
• FTA paper protocol
• Salicylic acid (SA) extraction protocol
• Arabidopsis thaliana quick seeds sterilization
• Total protein extraction
• Chart for SDS PAGE
• Plasma membrane prepration
• Western procedure
• Poly(A) RNA in situ localization
• KIDS-DNA extraction