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Portail UQTR
Bottin
Université du Québec à Trois-Rivières
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Canada Research Chair in Plant Immunity
Functional Genomics of Plant Pathosystems
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Hugo Germain Ph.D.
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Affiliation
KIDS-DNA extraction
Material
Fruit such as bananas
Table Salt
Baking soda
Liquid dishsoap
Rubbing alcohol
Cheese cloth (or coffee filter)
Tooth picks or paper clip
Ice bucket
Procedure
Place alcohol in ice bucket and keep for later
Prepare “DNA extraction solution”: add 1 tsp baking soda, 1 tsp dish soap and ¼ tsp salt to ½ cup of water in a becher.
Put 1 1-inch slice of fruit in a small bowl and smash with a spoon or mortar and pestle.
Add 3-4 tbsp “DNA extraction solution” to the bowl and grind with the spoon until the fruit is well pulverized.
Place the cloth on top of a narrow becher. Filter the DNA extract.
Add an equal volume of the cold rubbing alcohol, mix by swirling. Wait ~ 5 min. or more.
“Fish” out precipitated DNA with a paper clip hook.