Functional Genomics of Plant Pathosystems

Chart for SDS PAGE

Volumes for X% of acrylamide gel from a 30% stock solution
%	Volume (mL)	5	10	15	20	25	30	40	50
6%	H₂0	2671	5342	8013	10684	13355	16026	21368	26710
	30% Acrylamide	1000	2000	3000	4000	5000	6000	8000	10000
	1,5M Tris-HCl 8.8	1250	2500	3750	5000	6250	7500	10000	12500
	20% SDS	25	50	75	100	125	150	200	250
	10 % APS	50	100	150	200	250	300	400	500
	TEMED	4	8	12	16	20	24	32	40
8%	H₂0	2339	4677	7016	9355	11693	14032	18709	23387
	30% Acrylamide	1333	2667	4000	5333	6667	8000	10667	13333
	1,5M Tris-HCl 8.8	1250	2500	3750	5000	6250	7500	10000	12500
	20% SDS	25	50	75	100	125	150	200	250
	10 % APS	50	100	150	200	250	300	400	500
	TEMED	3	6	9	12	15	18	24	30
10%	H₂0	2006	4013	6019	8025	10032	12038	16051	20063
	30% Acrylamide	1667	3333	5000	6667	8333	10000	13333	16667
	1,5M Tris-HCl 8.8	1250	2500	3750	5000	6250	7500	10000	12500
	20% SDS	25	50	75	100	125	150	200	250
	10 % APS	50	100	150	200	250	300	400	500
	TEMED	2	4	6	8	10	12	16	20
12%	H₂0	1673	3346	5019	6692	8365	10038	13384	16730
	30% Acrylamide	2000	4000	6000	8000	10000	12000	16000	20000
	1,5M Tris-HCl 8.8	1250	2500	3750	5000	6250	7500	10000	12500
	20% SDS	25	50	75	100	125	150	200	250
	10 % APS	50	100	150	200	250	300	400	500
	TEMED	2	4	6	8	10	12	16	20
15%	H₂0	1173	2346	3519	4692	5865	7038	9384	11730
	30% Acrylamide	2500	5000	7500	10000	12500	15000	20000	25000
	1,5M Tris-HCl 8.8	1250	2500	3750	5000	6250	7500	10000	12500
	20% SDS	25	50	75	100	125	150	200	250
	10 % APS	50	100	150	200	250	300	400	500
	TEMED	2	4	6	8	10	12	16	20
Stacking	H₂0	692	1385	2077	2769	3462	4154	5539	6923
	30% Acrylamide	167	333	500	667	833	1000	1333	1667
	1,5M Tris-HCl 6.8	125	250	375	500	625	750	1000	1250
	20% SDS	5	10	15	20	25	30	40	50
	10 % APS	10	20	30	40	50	60	80	100
	TEMED	1	2	3	4	5	6	8	10